Callus culture from a carrot explant is the process of growing an unorganized mass of undifferentiated carrot cells (callus) on a nutrient medium in a sterile laboratory environment. This is initiated by taking a small piece of tissue (explant) from a carrot, sterilizing it, and placing it on a specially formulated agar medium containing plant hormones like auxin and cytokinin to stimulate cell division. This callus can then be used for various biotechnology applications, such as cloning plants
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